4D cell imaging allows to study dry mass production rate and the dry mass surface density in wild-type and mutant fission yeast cells. It demonstrates the applicability of DHM® as a tool for label-free quantitative analysis of the cell cycle.
Quantitative Phase Microscopy (QPM) solution by DHM® enables to study the dry mass production rate and dry mass surface density through the cell cycle of wild-type and mutant yeasts at the single-cell level in a non-invasive manner. It shows that the dry mass production rate of the mutant and wild-type cells are significantly different, and the wild-type dry mass surface density shows a specific pattern resulting from the cytokinesis process, which is absent in the mutant. Dry mass accumulation can be combined with other parameters easily measured by DHM®, e.g. the volume and refractive index, to provide a better understanding of the cell cycle.
Quantitative Phase Microscopy (QPM) solution by DHM® is used for cell analysis and recognition. A practical application is demonstrated on pollen particles.
The ability of the QPM solution for cell analysis and recognition is demonstrated for pollen particles cells identification. It could be applied to other type of cells, not only vegetable but also mammalian.